Title Echinops sphaerocephalus and Chrysochromulina polylepis algae fractionation, analysis and evaluation /
Translation of Title Apskritagalvio bandrenio Echinops sphaerocephalus ir Chrysochromulina polylepis dumblių frakcionavimas, analizė ir įvertinimas.
Authors Raškauskas, Paulius
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Pages 74
Keywords [eng] Echinops sphaerocephalus ; Chrysochromulina polylepis ; analysis
Abstract [eng] This master thesis consists of two parts. The aim of the first part of the thesis was to determine fatty acid composition, total phenolic content and antioxidant activity of Echinops sphaerocephalus extracts made with three different polarity solvents – hexane, acetone and methanol (70:30 %), as well as for extraction residue. Also to perform its supercritical fluid extraction optimization. Fatty acid composition was determined using gas chromatography. Total phenolic content was determined using Folin-Ciocalteu method. The antioxidant activity of extracts was determined using DPPH•, ABTS+• and ORAC assays. The results showed that mostly unsaturated fatty acids compose the oil, the phenolic content of the extracts were dependent on the extraction solvent namely that methanol extracts possessed most of phenolic content and antioxidant activity compared to other two. The residue tests showed opposite results that hexane residue contained more phenols and had more antioxidant activity compared to other extracts. Along those tests optimal supercritical fluid extraction parameters were determined with which to obtain best oil yield. The aim of the second part of the project was to determine best extraction mode of unidentified target compound of Chrysochromulina polylepis using solid phase extraction columns of different polarity and ionization and different solvents of different polarity and then to determine whether that compound had toxic effects on sea life or not using fish gill assay. Small scale extractions showed that the best target compound yield was using either acetone or methanol solvents with strong anion exchange column however due to large salt content when upscaled to large scale it was proven ineffective. C18 column was then chosen which provided nearly as good yield as SAX column on small scale tests. The fraction containing the target compound was then tested for toxicity but was proven to be non-toxic. However other toxic fractions were isolated and possible toxic compounds detected.
Dissertation Institution Kauno technologijos universitetas.
Type Master thesis
Language English
Publication date 2015