| Abstract [eng] |
Electrospinning is one of the used techniques in textile engineering industry, that forms nonwoven mat from nano-microfibers with different kinds of properties that depends on the type of selected polymers, which is a big variety to choose. It is possible to successfully and efficiently incorporate drugs and bioactive materials into nano-microfibers formed by electrospinning technique, their release and delivery can be controlled and targeted straight into the inflammation place. Mixtures of synthetic polymers ensures stability of nano-microfibers together with mechanical properties as well as maintains biomedical properties, avoids too rapid desorbtion or degradation of the molecules. In this Master’s Final Degree Project pharmaceutical Poly (methyl methacrylate) (PMMA) derivatives Eudragit S-100 (ES), Eudragit E-100 (EE) and polyvinylpyrrolidone (PVP) polymers that offer antibacterial, good mechanical properties as well as mixtures in 50/50 and 75/25 concentration dissolved in ethanol were chosen to form nano-microfibers by electrospinning technique. Furthermore, nonpsychotropic hemp extract was incorporated into the structure of nano-microfibers that has a scientifically proven great potential in a medical field and comparison of formed structures have been done during the investigation. Cannabis Sativa L. (Cannabigerol) is composed from more than 120 identified cannabinoid compounds, from which the most in quantity are nonpsychotropic cannabidiol (CBD) and cannabigerol (CBG) and that has anti-inflammatory and anti-bacterial properties. As hemp extract for electrospinning technique have been not investigated yet, this Master’s Final Degree Project task was to form solutions from biomedical polymers with hemp extract and investigate its properties, electrospin nonwoven materials from PVP, ES, EE with incorporated hemp extract, investigate the influence of biomedical polymer solution composition on a structure of electrospun solution with Scanning Electron Microscopy (SEM), investigate the release kinetics of hemp components from electrospun solution and analyze the chemical composition of electrospun nonwoven materials. Results demonstrated no significant changes on viscosity of solution after adding hemp extract, however electrical conductivity and Ultraviolet-Visible Spectroscopy (UV-VIS) test results demonstrated values that confirm the presence of hemp extract in a polymeric solution and electrospun nonwoven respectively, also release of hemp extract was determined from one-component nonwoven. The obtained results can testify that hemp extract can be incorporated into the biomedical polymer solutions and electrospun into a nano-microfiber nonwoven that can be used for biomedical purposes. |
