| Abstract [eng] |
Increasing number of people is the driving force for growing demand for food, which is however not being accompanied by increase in food supply. Rapeseed is the second most extensively grown oilseed crop. However, due to presence of considerable amount of toxic and anti-nutritive factors such as glucosinolates, phytates, and phenols, which reduces its capacity to serve as potential protein source to humans. Hence, aim of the project is to modify rapeseed press cake by enriching protein fractions by air-classification. The physiochemical properties of air classified samples along with RPF, RPC and concentrates were determined. The percent protein solubility of samples were analysed over a pH range of 1-10 and it has been observed that protein solubility increases on moving away from the isoelectric point (pH 3-5). The water binding capacity (WBC) and fat binding capacity (FBC) of samples were found to be negatively related. In second part of the project, protein solubility of Rapeseed press cake was studied under the influence of digestive enzymes of T.molitor larvae (subjected to starvation for 0hrs, 24 hrs and 48 hrs) were cake in combination with extrusion. Protein solubility has decreased considerably due to formation of disulphide linkages which has resulted in protein aggregation while extrusion. |
