Title Integration of silver nanoparticle-polymer nanocomposite into 3D-printed protective cover: a study of antiviral performance /
Authors Ilickas, Mindaugas ; Guobienė, Asta ; Gedvilas, Karolis ; Merkis, Mantvydas ; Abakevičienė, Brigita
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Is Part of ANM2024: conference on advanced nano materials, 24-26 July 2024, University of Aveiro, Portugal: abstract book.. Aveiro : University of Aveiro. 2024, p. 124
Keywords [eng] silver nanoparticles ; photochemical synthesis ; antimicrobial coatings ; qRT-PCR analysis
Abstract [eng] Efforts to combat microorganisms involve surface modification and the development of antimicrobial coatings. Direct deposition of biocidal substances on surfaces enables effective modification without altering bulk properties. Recent advancements include polymer-solvent-active material nanocomposites1, providing varied antimicrobial effects based on concentrations and compound compositions. The use of 3D scanning and printing allows to produce complex, geometrically shaped, and flexible protective coatings2 to mitigate the spread of microorganisms on frequently touched surfaces. In this work, AgNPs synthesized through photochemical methods3 using AgNO3 and Irgacure 819 are incorporated into a PVB polymer matrix, forming an AgNP-PVB nanocomposite. This nanocomposite is applied as a thin-film coating on custom protective cover produced with Artec Space Spider 3D scanner (Artec 3D, Luxembourg) and Form 3 stereolithographic 3D printer (Formlabs, USA) using Flexible 80A resin (Formlabs, USA). The 3D model reconstruction algorithm is developed using Matlab (MathWorks, USA). To evaluate the antiviral properties, 10-well substrates were 3D printed from Flexible 80A polymer, 8 wells were coated with AgNP-PVB-A (AgNP concentration – 500 ppm), and AgNP-PVB-B (AgNP concentration – 200 ppm) nanocomposites or were left empty to assess the properties of the printed cover itself. The 8 wells are then filled with the test solution and left for 24 hours. The 9th and 10th wells are used as controls and are filled with the test solution for the one-step qRT-PCR analysis after 24 hours. The results of the antiviral tests using the AgNP-PVB-A nanocomposite coating show that the average cycle threshold (Ct) value in the test wells is 30.78 ± 2.00 compared to 25.92 ± 0.04 in the control wells. The Ct values decrease to 28.22 ± 0.88 (test) and 24.65 ± 0.40 (control) when using the AgNP-PVB-B nanocomposite coating. The AgNP-PVB-B control test Ct values are similar to those of the Flexible 80A polymer (25.27 ± 1.41 (test) and 24.61 ± 0.11 (control)), indicating the antiviral properties of the printed coating itself. The 3D printed door handle cover showed a uniform thickness with no cracks or bulges. This research contributes to the development of durable antiviral coatings aimed at inhibiting the transmission of infectious diseases in various environments. In conclusion, this study formed different concentration (AgNP concentration – 200 ppm and 500 ppm) AgNP-PVB nanocomposite coatings on 3D printed protective cover, demonstrating effective antiviral properties, with Ct values of 30.78 ± 2.00 and 28.22 ± 0.88. Also, an algorithm for reconstructing the 3D model of the cover from the 3D scanned model has been developed and tested.
Published Aveiro : University of Aveiro
Type Conference paper
Language English
Publication date 2024