| Abstract [eng] |
Targeted alpha therapy (TAT) shows promising results for precise and selective radiotherapy with effective cancer treatment and minimal toxicity. Alpha particle emitters are linked to the targeted molecules for the selective delivery of ionizing radiation to the malignant cells, and due to the short path length (~65 μm), only a few cells are affected. A short distance of irradiation ensures minimal toxicity for the healthy cells around and provides the possibility to treat very small targets—groups of cells. In combination with less selective treatments, targeted alpha therapy could provide higher treatment quality with a decreased probability of cancer recurrence. However, targeting agents, dose regiments, and possible alpha particle emitters raise questions that should be answered. This project aimed to evaluate the effect of different doses of alpha (223Ra) and gamma (6 MeV photons) radiation on the size and viability of glioblastoma and prostate cancer 3D cell cultures and provide recommendations for future experiments. The size of cell structures was evaluated on the 7th and 14th-day post-irradiation, and the effect on viability was analyzed on the 14th day post-irradiation. It was found that with increased specific activity of the alpha particle emitter 223Ra solution both 3D cell cultures showed a decrease in size. The same effect was observed after irradiation with photons. The viability test showed a significant positive correlation between spheroid size and viability, indicating the possibility of determining spheroid viability from the size measurements. |
