| Abstract [eng] |
The aim of the study: to model and evaluate different polymer film compositions for microneedles as a drug delivery system, its use, efficient drug delivery (lidocaine) and dermal penetration. The object of the work: polymer films and microneedles coated with them for drug supply. Relevance of the work: the aim to improve the delivery of compounds to the body through the skin using microneedles, which can more efficiently and effectively transfer drugs while maintaining their biological activity. Tasks: to model polymer film compositions for microneedles and to select them according to the film appearance, elasticity, brittleness and mechanical properties. Coat microneedle frames with selected polymer film compositions and evaluate coating quality. To evaluate the release of the drug substance (lidocaine) from the polymer film by in vitro studies. To investigate the penetration of the drug substance (lidocaine) into the skin ex vivo. Results of the work: The composition (1.5 % carboxymethylcellulose, 5 % polyvinyl alcohol, 5 % glycerol) was chosen from the simulated polymer film compositions because it remains unchanged for two months. Exposure of the skin with microneedles coated with a polymeric composition (1.5 % carboxymethylcellulose, 5 % polyvinyl alcohol, 5 % glycerol) and 7 % lidocaine, and ex vivo studies, showed that lidocaine levels were higher in the dermis than in the epidermis. The use of lidocaine-coated microneedles with polymer films revealed a low amount of penetrated lidocaine compared to the amount released in the in vitro study 24.35 μg (97.34 %). Given the limited amount of hydrophilic phase in the biological matrix, this can be considered as a limiting factor for the dermal penetration of lidocaine ex vivo. The proposed way to increase the amounts of lidocaine in the skin layers is to use polymeric coatings with higher amounts of the drug substance. Conclusions: The obtained research results provided additional insights into the penetration of lidocaine using polymer-coated microneedles as penetration-promoting systems. It was found that the application of a polymeric composition with a drug substance (lidocaine) to the skin with microneedles resulted in a 3.72-fold higher penetration of lidocaine into the dermal layer compared to the control. |
