| Abstract [eng] |
Hypericum perforatum L. has long been used as a medicinal plant with antioxidant, antibacterial, anti-inflammatory and antidepressant properties. St. John’s wort is rich in bioactive compounds such as hypericin, hyperforin, pseudohypericin, flavonoids and other compounds, which determine these properties. Hypericum medications are widely used in medicine, pharmacy, regular research is carried out to determine and evaluate antioxidant and antibacterial activity. The most effective way to study this is to use a variety of growth-determining hormones that are responsible for plant growth, development, cell division and differentiation, and proteins that are important for the immune response. The use of these substances attempts to accelerate and improve plant growth and increase the concentrations of bioactive compounds [1]. During the study, different media were prepared, which differed in their composition - auxins (2,4-D, NAA) and / or cytokines (BAP, kinetin) were added, different concentrations were used. H. perforatum L. callus cultures were formed in vitro, and the antioxidant and antibacterial activity of their extracts was evaluated. Antioxidant activity was assessed using reductive, FRAP, DPPH and ABTS methods, antibacterial activity - using agar diffusion method. The influence of 39 compound (N-(1,3-dioxoisoindolin-2-yl)-3-((4-methoxyphenyl)amino)propanamide) and the part of the plant explant from which callus cultures were grown on the antioxidant and antibacterial activity of St. John’s wort was evaluated. Catalase and SOD activity, the content of phenolic compounds, MDA, lutein, chlorophyll a and b and carotenoids, flavonoids were determined, as well as the influence of 39 compound’s and plant explant parts from which were grown on these parameters was evaluated. Proline dehydrogenase and ascorbate peroxidase activity, proline and protein content were examined. A technological scheme for the extraction of hyperforin from H. perforatum L. callus cultures by in vitro extraction using supercritical CO2 was proposed. |
