| Abstract [eng] |
During research, the production of carotenoids from different yeasts were analyzed. Three different media were used for the selection of dye-producing yeasts – traditional yeast extract / peptone / glucose, whey, molasses and the influence of cell growth time on cell growth, biomass and carotenoid formation was evaluated. The highest amount of carotenoids were found to be formed by growing yeast in a traditional medium. In whey medium, most carotenoids were formed in samples containing Sporobolomyces bannaensis yeast. Growing yeast cultures under research in molasses medium, the highest amounts of biomass and carotenoids were found in the samples with yeast strains of Rhodotorula glutinis. Other yeast cultures in molasses medium have accumulated small amounts of carotenoids. Based on the results of the research, two yest cultures were selected to carry out further research – Rhodotorula glutinis RY and Sporobolomyces bannaensis DU. The ability of these yeasts to produce carotenoids at different glucose concentrations of 20, 30, 40 and 50 g/l has been evaluated. Research was carried out by changing the concentration of yeast extract as a nitrogen source – 10, 20, 30 and 40 g/l. The effect of NaCl ir MgSO4·7H2O salts on carotenoid formation was evaluated. Different media of 10, 30 and 60 g/l concentration and different media of (2.5, 5 and 7.5 g/l) MgSO4·7H2O concentration were prepared. The antioxidant activity of carotenoid-producing yeast was evaluated using 2.2-diphenyl-1- picrylhydrazyl (DPPH•) and 2,2’-azino-bis-(3-enthylbenzothiazoline-6-sulfonic acid) (ABTS+) methods. The antimicrobial activity of carotenoid extracts was determined using 7 different pathogenic microorganisms using the diffusion agar method. The hardware scheme of carotenoid production was drawn. |
