| Abstract [eng] |
Bioactive polymeric dressings are one of the most effective materials for wound healing. Dressings are formed from natural or synthetic polymers or their mixtures and for functionalization bioactive components are immobilized what provides polymeric film dressing anti-inflammatory, antibacterial, analgesic or tissue regeneration properties. Bioactive film base dressings for wound healing were formed during this project. 18 compositions of wound dressings were created. It has been determined that compositions should contain: 0.2-0.4% hydroxyethylcellulose, 0.1-0.3% pectin, 0.05-0.1% hyaluronic acid, 0.3% D-panthenol and water up to 100% because otherwise – film base dressing does not form. It was found that the percentage of hydroxyethylcellulose and pectin in compositions had the biggest effect on thickness, transparency, moisture content and mechanical properties of the dressings. As the thickness of the polymer dressing increases, the moisture content in them increases too and the dressings are more elastic and less transparent to light. For dressings functionalization several active components were selected: octenidine dihydrochloride, lidocaine hydrochloride and licorice root extract. It has been determined, that solutions for polymer dressings should contain 0.2-0.3% hydroxyethylcellulose, 0.2-0.3% pectin, 0.2% polyvinylpyrrolidone, 0.05-0.1% hyaluronic acid, 0.3% D-panthenol, 0.025-0.5% active components and water up to 100%. Thin and sufficiently transparent polymer wound dressings with bioactive components were formed. The thickness of bioactive dressings ranged from 0.09 ± 0.01 mm to 0.12 ± 0.01 mm and the light transmittance was 79-94%. In vitro release kinetics of the active compounds showed that complete release of lidocaine hydrochloride from the dressing was achieved after 6 hours. Meanwhile, only 92% of octenidine dihydrochloride was released due to the formation of a complex with hyaluronic. The antibacterial activity of polymer dressings was tested against Gram-negative Escherichia coli bacteria. The wound dressing with octenidine dihydrochloride was found to have the strongest antibacterial effect, the inhibition zone against Escherichia coli bacteria was the largest and reached up to 14 ± 1 mm. |
