| Abstract [eng] |
Biotechnological processes, types of parameters and measuring methods of different variables are reviewed in this work. Currently existing soft sensors, their types and measuring parameters are examined. Modern industrial aeration system of bioreactor, various balance equations and mathematical models are studied as well. A real and operative bioprocess using a batch bioreactor was chosen for the research. Experiments were carried out and gas concentrations, flows, as well as measured amounts of various materials supplied for bioreactor and data about biomass concentrations measured using an off-line method, were collected. Using balance equations, mathematical models and the data collected, the soft sensors for indirect evaluation of biomass concentration are created. By using various on-line directly measured process variables (concentrations, flows of O2 supply and emitted CO2, etc.) they evaluate the values of biomass concentration indirectly and on-line. Two different methods for evaluation of biomass concentration were used. One of them was measuring of oxygen uptake rate (OUR) and the other – carbon dioxide production rate (CPR) measurement. A testing of the created soft sensors was carried out, as well as a quality check of the simulation. Finally, the identification of model parameters was carried out, which led to even more accurate results of biomass evaluation, accordingly ensuring the effectiveness of the methods used. The identification of parameters was performed by means of digital Chemotaxis random search procedures. |
