Title Synthesis, ADMET properties, and in vitro antimicrobial and antibiofilm activity of 5-nitro-2-thiophenecarbaldehyde N-((E)-(5-nitrothienyl)methylidene)hydrazone (KTU-286) against Staphylococcus aureus with defined resistance mechanisms /
Authors Kavaliauskas, Povilas ; Grybaite, Birutė ; Mickevicius, Vytautas ; Petraitiene, Ruta ; Grigaleviciute, Ramune ; Planciuniene, Rita ; Gialanella, Philip ; Pockevicius, Alius ; Petraitis, Vidmantas
DOI 10.3390/antibiotics9090612
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Is Part of Antibiotics.. Basel : MDPI. 2020, vol. 9, iss. 9, art. no. 612, p. 1-17.. ISSN 2079-6382
Keywords [eng] bisnitrothiophene ; Staphylococcus aureus ; MRSA ; VRSA ; antimicrobial ; small molecules ; thiophene derivatives ; drug resistant ; hydrazone
Abstract [eng] The emergence of drug-resistant Staphylococcus aureus is responsible for high morbidity and mortality worldwide. New therapeutic options are needed to fight the increasing antimicrobial resistance among S. aureus in the clinical setting. We, therefore, characterized the in silico absorption, distribution, metabolism, elimination, and toxicity (ADMET) and in vitro antimicrobial activity of 5-nitro-2-thiophenecarbaldehyde N-((E)-(5-nitrothienyl)methylidene)hydrazone (KTU-286) against drug-resistant S. aureus strains with genetically defined resistance mechanisms. The antimicrobial activity of KTU-286 was determined by CLSI recommendations. The ADMET properties were estimated by using in silico modeling. The activity on biofilm integrity was examined by crystal violet assay. KTU-286 demonstrated low estimated toxicity and low skin permeability. The highest antimicrobial activity was observed among pan-susceptible (Pan-S) S. aureus (minimal inhibitory concentration (MIC) 0.5–2.0 µg/mL, IC50 = 0.460 µg/mL), followed by vancomycin resistant S. aureus (VRSA) (MIC 4.0 µg/mL, IC50 = 1.697 µg/mL) and methicillin-resistant S. aureus (MRSA) (MIC 1.0–16.0 µg/mL, IC50 = 2.282 µg/mL). KTU-286 resulted in significant (p < 0.05) loss of S. aureus biofilm integrity in vitro. Further studies are needed for a better understanding of safety, synergistic relationship, and therapeutic potency of KTU-286.
Published Basel : MDPI
Type Journal article
Language English
Publication date 2020
CC license CC license description