| Title |
Low-frequency (20 kHz) ultrasonic modulation of drug action / |
| Authors |
Abramavičius, Silvijus ; Volkevičiūtė, Augusta ; Tunaitytė, Agilė ; Venslauskas, Mantas ; Bubulis, Algimantas ; Bajoriūnas, Vytis ; Stankevičius, Edgaras |
| DOI |
10.1016/j.ultrasmedbio.2020.07.002 |
| Full Text |
|
| Is Part of |
Ultrasound in medicine & biology.. New York : Elsevier. 2020, vol. 46, iss. 11, p. 3017-3031.. ISSN 0301-5629. eISSN 1879-291X |
| Keywords [eng] |
Calcium signaling ; Drug action ; Insonation ; Low-frequency ultrasound ; Ultrasonic modulation |
| Abstract [eng] |
We tested the effect of low-frequency ultrasound (LUS, 20 kHz, 4 W/cm2) on the function of rat mesentery and human pulmonary arteries with wire myography. The vessels were induced to contract with either noradrenaline or physiologic saline solution (PSS) with a high potassium concentration (KPSS) and then incubated with capsaicin (2.1 × 10-7 M, TRPV1 [transient receptor potential vanilloid 1] activator), dopamine (1 × 10-4 M, dopamine and α2-receptor activator), or fenoldopam (dopamineA1 receptor agonist, 1 × 10-4 M) with and without glibenclamide (1 μM, KATP [adenosine triphosphate {sensitive potassium channel (ATP)}-sensitive potassium channel] inhibitor and α2-receptor modulator), and insonated. Vessels were incubated in Ca2+-free PSS and induced to contract with added extracellular Ca2+ and noradrenaline. Pulmonary arteries were induced to contract with KPSS and dopamine. Then the vessels were insonated. LUS inhibited the influx of external Ca2+, inhibited the dopamine-induced vasoconstriction in the KPSS (glibenclamide reversible), reduced the capsaicin-induced vasorelaxation, increased the gentamicin-induced vasorelaxation and increased the dopamine-induced contraction in the KPSS in human pulmonary arteries. |
| Published |
New York : Elsevier |
| Type |
Journal article |
| Language |
English |
| Publication date |
2020 |
| CC license |
|
