Title Kiekybinis bendrųjų fenolinių junginių, flavonoidų, antioksidacinio ir antibakterinio aktyvumo įvertinimas Astrinių (lot. Asteraceae) šeimos augalų dalyse in vivo ir kaliaus kultūrose in vitro /
Translation of Title Evaluation of total phenolic compounds, flavonoids quantities, antioxidant and antibacterial activities in Asteraceae family plant parts in vivo and callus cultures in vitro.
Authors Skujytė, Iveta
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Pages 64
Keywords [eng] Asteraceae ; phenolic compounds ; antioxidants ; antibacterials ; bioactivities
Abstract [eng] In this project, the concentrations of total phenolic compounds, flavonoids were determined, antioxidant activities and antibacterial effects in parts of Astrid family plants (marigold, purple coneflower and chamomile) in vivo and in callus cultures in vitro were investigated. The research showed that marigold flower extract in vivo had the highest reductive properties (0.793 A). The in vivo extracts of marigold (0.239 mol / l) and purple coneflower (0.223 mol/l) flowers showed the highest properties of the antioxidant activity of extracts of plants grown in vivo and in vitro by FRAP method. During the DPPH detection of antioxidant activity, it was observed that the strongest antioxidant properties were in purple coneflower flowers in vivo extract (92.9 %). The highest concentrations of phenolic compounds were found in the in vivo extract of purple coneflower flowers (2.98 mg / 100 mg) and in flavonoids in the in vivo extract of marigold flowers (15.318 mg/g). The highest activity of superoxide dismutase enzyme was found in in vitro extract of marigold cultures (58.47 units/mg). The highest concentration of the L-proline was detected in the in vivo extract of chamomile flowers (1.386 mol / g). The highest concentration of chlorophyll a was found in in vivo extracts of calendula stems (180.65 mg / 100 mg), chlorophyll b in chamomile flowers in vivo (364.54 mg / 100 mg), and carotenoids in in vivo extracts of marigold flowers (847.745 mg/100 mg). The highest concentration of lutein was found in the in vivo extract of calendula flowers (1.096 mg/g). The strongest antibacterial activity was observed in the in vivo extracts of chamomile plant parts, which most intensively inhibited the growth of Escherichia coli and Bacillus subtilis bacteria.
Dissertation Institution Kauno technologijos universitetas.
Type Master thesis
Language Lithuanian
Publication date 2020