||In response to recent ecological solutions nowadays and in order to reduce the pollution of material production of materials, and make the fashion business more sustainable, designers and researchers are increasingly interested in biodegradable, environmentally friendly materials. The exclusive production, structure and characteristics of the bacterial cellulose film formed on the surface of the Kombucha drink inspires developers and scientists to draw more attention and interest in this material. In order to apply this popular bacterial cellulose material in production of clothing, it is necessary to study the properties of the material which are important by wearing a product. In final work, bacterial cellulose (BC) film formed by fermentation caused symbiotic cultures of Zygosaccharomycea bailii yeast and Komagataeibacter rhaeticus acetic acid bacteria, is investigated. The aim of the research is to investigate the influence of preparation and chemical treatment on the film thickness and its distribution, mechanical properties of the bacterial cellulose membrane, also to determine the spatial formation ability of the film and to evaluate its potential application as an alternative material in the clothing industry. The performed results showed that washing procedure and chemical treatment with different concentrations (5, 10 and 20%) of DMDHEU reagent of BC film has impact on thickness, density and surface uniformity of the material. During the experiment was found that chemically treated bacterial cellulose film has a greater thickness uniformity in the sample area – after drying procedure the variation of the thickness value decreased from 15.10 to 13.39%. According to the experiment results, the highest amount of bending stiffness is determinated for non-washed dried BC film sample (B = 28,79 μNm2), while the lowest bending stiffness is tested for 5% DMDHEU treated sample (B = 3.15 μNm2). It was calculated that tensile strength of tested BC membrane specimens ranges from 15.47 MPa to 42.38 MPa when elongation at break varies from 2.54% to 34.1%, respectively. The original methodology testing the properties of formation of BC material was selected and applied in the final work. Experiment has shown that dried an affected by external force BC film is suitable for enshrouding smoothly on a selected spherical surface; the formed spatial shape is stabile and does not change during the observed time period. It has been found that the spatial shape can be formed from wet BC film and the area of the enshrouded surface increases after the drying procedure. Performed results showed that the biggest area of enshrouded spherical surface, 100% and 64,67%, is measured respectively for a thin (average thickness 0,87 mm) and a thick (average thickness 1,43 mm) 120 mm size BC samples.