Title Augimo fitohormonų ir salicilo rūgšties įtaka jonažolės (lot. Hypericum perforatum L.) kaliaus kultūrų in vitro susiformavimui ir biologiniam aktyvumui /
Translation of Title The influence of growth phytohormones and salicylic acid on Hypericum perforatum callus cultures formation in vitro and biological activity.
Authors Milašauskaitė, Karolina
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Pages 55
Keywords [eng] Hypericum perforatum ; in vitro ; callus cultures ; salicylic acid ; bioactivity
Abstract [eng] The object of research of this final project was St. John’s wort (Hypericum perforatum L.) callus cultures in vitro and St. John's wort in vivo. St. John's wort is one of the most popular medicinal plants used around the world. This plant is relevant for its exceptionally high bioactivity and therapeutic antidepressant properties. The fast growing pharmaceutical industry is promoting science research to obtain higher quality raw plant material which could have the same phytochemical profile. Therefore, ways are being sought to grow bioactive-enriched St. John's wort callus cultures in vitro which would be superior to St. John’s wort callus cultures in vivo in their bioactivity. The results of the final project – the research of St. John‘s wort in vitro which was grown with different phytohormones and different concentrations of elicitor – salicylic acid and St. John's wort in vivo were made. The results of antioxidant activity, activity of antioxidant enzymes were evaluated as well as the amount of malondialdehyde and the amounts of biologically active compounds - carotenoids, phenolic compounds and L-proline were determined. The conclusions obtained during the final project showed that St. John's wort callus cultures in vitro which were grown in MS medium with growth phytohormones 2,4-D (0.1 mg/l) and BAP (0.5 mg/l) as well as St. John‘s wort root callus cultures which were cultivated with 1 mM salicylic acid had the highest antioxidant activity. The greatest activity of antioxidant enzyme superoxide dismutase (8.67 unit/mg) showed St. John's wort callus cultures in vitro which were grown with growth phytohormones BAP (2.5 mg/l) and NAA (0.5 mg/l) and the greatest activity of catalase (5.68 unit/mg) was found in St. John‘s leaves callus cultures in vitro, which were grown with 2.5 mM salicylic acid. It was found that the highest amount of carotenoids (19.23 mg/100g) compared to St. John‘s wort callus cultures in vitro that were grown with salicylic acid, was in the St. John‘s wort root callus cultures which were grown with 5 mM salicylic acid. It was found that the highest amount of phenolic compounds (6.13 mg/100g) were in St. John‘s wort callus cultures in vivo. The highest L-proline content (5.97 μmol/g) was found to be in vitro callus cultures of St. John's wort grown with TDZ (0.05 mg/l) and NAR (0.2 mg/l) and salicylic acid showed the best influence on L-proline formation of St. John's wort leaves callus cultures in vitro (8.53 μmol/g) which medium contained 2.5 mM of salicylic acid.
Dissertation Institution Kauno technologijos universitetas.
Type Master thesis
Language Lithuanian
Publication date 2020