| Title |
Haloperoxidase mediated quorum quenching by Nitzschia cf pellucida: Study of the metabolization of N-Acyl homoserine lactones by a benthic diatom / |
| Authors |
Syrpas, Michail ; Ruysbergh, Ewout ; Blommaert, Lander ; Vanelslander, Bart ; Sabbe, Koen ; Vyverman, Wim ; De Kimpe, Norbert ; Mangelinckx, Sven |
| DOI |
10.3390/md12010352 |
| Full Text |
|
| Is Part of |
Marine drugs.. Basel : MDPI. 2014, vol. 12, iss. 1, p. 352-367.. ISSN 1660-3397 |
| Keywords [eng] |
AHL ; degradation pathway ; diatom-bacteria interactions ; haloperoxidase ; quorum sensing ; reference compounds |
| Abstract [eng] |
Diatoms are known to produce a variety of halogenated compounds, which were recently shown to have a role in allelopathic interactions between competing species. The production of these compounds is linked to haloperoxidase activity. This research, has shown that this system may also be involved in diatom-bacteria interactions via the H2O2 dependent inactivation of a type of quorum sensing (QS) molecule, i.e., N-β-ketoacylated homoserine lactones (AHLs), by a natural haloperoxidase system from the benthic diatom Nitzschia cf pellucida. The AHL degradation pathway towards corresponding halogenated derivatives was elucidated via HPLC-MS analysis and the synthesis of a broad series of novel halogenated AHL analogues as reference compounds. Furthermore, their biological activity as quorum sensing modulators was directly compared and evaluated against a series of naturally occurring β-keto-AHLs. It has been demonstrated that the loss of the QS activity results from the final cleavage of the halogenated N-acyl chain of the signal molecules. © 2014 by the authors licensee MDPI. |
| Published |
Basel : MDPI |
| Type |
Journal article |
| Language |
English |
| Publication date |
2014 |
| CC license |
|
