| Abstract [eng] |
Potentilla fruticosa is a shrubby perennial plant, native to Northern areas of America and Europe. It is commonly consumed as an herbal tea and exhibits great antioxidant potential as well as antifungal, antibacterial and anti-inflammatory properties. Hierochloe odorata is an aromatic perennial grass, widely distributed in West Asia and Europe, whose roots and aerial parts have a sweet smell. H. odorata extracts have shown to have high antioxidant activity and insect-repellent properties. The aim of this work was to isolate and analyze functional characterization of high-added value fractions of P. fruticosa and H. odorata through the development of biorefining schemes utilizing conventional, high-pressure, and enzymatic treatment techniques. The chemical composition of P. fruticosa and H. odorata was studied using various basic methods. Conventional and high-pressure extraction techniques like Solid liquid extraction and supercritical carbon dioxide extraction, pressurized liquid extraction and enzyme assisted extraction were applied on P. fruticosa and H. odorata. Defatted P. fruticosa and H. odorata residues after supercritical carbon dioxide extraction were used for further extraction techniques. PLE conditions were optimized using response surface methodology to maximize the extraction yield and phenolic content. Enzyme assisted extractions of defatted plant material and water residue from PLE of P. fruticosa and H. odorata were performed. Total phenolic content was determined using Folin-Ciocalteu’s technique and antioxidant activity was measured by DPPH• and ABTS•+ assays. Initial solid plants materials, SLE and SFE-CO2 residue antioxidant activity were analyzed using QUENCHER procedure. SLE, SFE-CO2, PLE, enzyme treated extracts total phenolic content was determined using Folin-Ciocalteu’s technique. Antioxidant activity was measured by DPPH• and ABTS•+ assays. The phytochemical characterization of volatile compounds of P. fruticosa and H. odorata SFE-CO2 extracts were analyzed by GCxGC/TOF MS, chemical compounds of P. fruticosa and H. odorata PLE extracts were analyzed using UPLC/ESI-QTOF-MS/MS. |
