| Abstract [eng] |
Forest trees account for the largest share of biomass in the world, but due to high demand for energy production and the wood industry, biomass resources are depleting and forest rotation is about 50 – 120 years. Which is why it has been started to look for faster ways to extract biomass in larger quantities. Short rotation forestry is an alternative way of solving this problem because this kind of forest’s rotation time is less than 30 years. This study was carried out to assess the suitability of hybrid aspen (Populus tremula ´ Populus tremuloides Michx.) for SRF (short rotation forestry). To select the most promising aspen hybrids and to identify their genetic diversity through molecular markers. During the studies, DNA was isolated from fresh leaves of hybrid aspen using CTAB method, its concentration was determined by a spectrophotometer. Nuclear DNA was amplified by PCR using random primers. The DNA fragments were analyzed by RAPD after they have been separated in agarose gel. The results of the study showed that out of the 53 analyzed oligonucleotide primers the most informative and highest amplification quality primers were: Roth B012, Roth B07, Roth 170 – 02, Roth 170 – 03 and Roth 170 – 10, the quality of amplification of other used primers was medium or low. Using the selected informative primers, 85.24% polymorphism between genotypes was detected, all hybrids were different. |
