| Abstract [eng] |
The final thesis aims at describing and analysing the following research issues related with pharmaceutically important plant Atropa belladonna, which accumulates tropane alkaloids scopolamine, atropine, hyoscyamine. Plants, naturally growing in the wild or cultivated under field conditions, usually accumulate only small amounts of alkaloids, because of the negative environmental impacts that are difficult to control. The aim of this work is to optimize lighting conditions for the cultivation of deadly nightshade (Atropa belladonna) in plant facotry, in order to obtain a stable and high quality raw material that accumulates large amount of valuable phytochemical compounds. In this work, the effects of illumination duration, intensity andspectral combinations on the growth rate, antioxidant properties, accumulated amount of alkaloids of Deadly nightshade were investigated. The results show that the plant growth parameters and the amount of alkaloids in the tissues can be tailored by adjusting the composition of the lighting parameters. Most of the scopolamine and atropine were accumulated within the highest lighting intensity (250 µmol m2 s-1) and 24 h photoperiod. Free radical scavenging activity, as well as the amount of alkaloids in the deadly nightshade, can be increased by illuminating them with sole red light. The antioxidant properties increases due to the reaction of the plant antioxidant protection system, while experiencing photostress, but also slightly reduce growth indices. When choosing lighting conditions in controlled environmental horticultural systems, it is important to seek for a balance between natural plant needs, technological aims and economic aspects. |
