| Abstract [eng] |
Alginate is a polysaccharide, widely used in important industrial areas. It is produced by the fermentation of Azotobacter vinelandii bacteria. There are used big amounts of alginate in industry, so it is necessary to increase the yield of this biopolymer. On purpose to improve production of alginate, the conditions of it‘s production are optimized. The sources of nutrients and their concentrations, also concentration of minerals in bacterial growth medium, are modified. In this research, there were changed carbon and nitrogen sources, their concentrations and concentration of mineral MgSO4. By making these changes it was posible to obtain optimal conditions of alginate production. There was also analyzed the content of carbohydrates in produced alginate and it‘s antioxidative effect. It was found that production of alginate is optimized, when as carbon source it is used maltose (30 g/L), as nitrogen source – yeast extract (6 g/L) and MgSO4 concentration is 3 %. In this case yield of alginate was 7.89 g/L. To obtain the maximum yield of alginate alternative carbon sources were also used. Sugar cane sugar (30 g/L) gave the highest amount of alginate (11.86 g/L). It was also obtained that alginate has the antioxidative effect. The highest antioxidative effect has alginate obtained using soy peptone as nitrogen source and the minimum quantity of carbohydrates has the alginate, which was obtained, when fructose was used as a carbon source. |
