| Title |
Natural C18:0-ceramide induces cellular sphingolipid accumulation and apoptosis / |
| Authors |
Babenko, Nataliya ; Hassouneh, Loay ; Budvytiene, Monika ; Liesiene, Jolanta ; Geilen, Christoph |
| DOI |
10.9754/journal.wmc.2010.001100 |
| Full Text |
|
| Is Part of |
Webmedcentral.. London : Webmed. 2010, vol. 1, iss. 11, art. no. WMC001100, p. 1-9.. ISSN 2046-1690 |
| Keywords [eng] |
ceramides ; sphingomyelin ; cytotoxicity ; proliferation ; apoptosis |
| Abstract [eng] |
Exogenous ceramides induces cell differentiation, cell cycle arrest and apoptosis in various cell types. However, the permeable short-chain ceramides have drawn more attention. In this study the effect of natural N-stearoyl-D-erythro-sphingosine (C18-ceramide) as well as short chain N-acetylsphingosine (C2-ceramide) and bovine brain non-hydroxy fatty acid ceramides on human keratinocyte growth and apoptosis and cellular sphingolipid accumulation was compared. Cells were cultered in the presence of 5-60 μM of ceramides or ethanol/dodecane for the 24 h. No significant release of lactate dehydrogenase was observed when the HaCaT cells were incubated with up to 60 μM of natural and C2-ceramides. Culturing of the cells in the presence of C18-ceramide leads to the concentration-dependent increase of the content of ceramide and sphingomyelin in the cells, reduction of keratinocyte proliferation, induction of cell apoptosis, and does not change the glucosylceramide content in the cells. It has been determined that the glucosylceramide synthase inhibitor D-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol alone as well as combined with C18-ceramide was not toxic for HaCaT cells and did not enhanced the C18-ceramide-iuduced keratinocyte apoptosis. Comparison of biological activity of the ceramides species showed that natural C18-ceramide is the most potent inhibitor of the HaCaT cell growth and an inducer of cellular sphingolipid accumulation and cell apoptotic death. |
| Published |
London : Webmed |
| Type |
Journal article |
| Language |
English |
| Publication date |
2010 |
| CC license |
|
