| Title |
Cytocompatibility assessment of amorphous calcium phosphates modified with tartaric or citric acid / |
| Authors |
Zhivkova, T ; Andonova-Lilova, B ; Abudalleh, A ; Dyakova, L ; Rabadzieva, D ; Tepavitcharova, S ; Saha, N ; Jankauskaitė, Virginija ; Alexandrova, R |
| DOI |
10.1002/2211-5463.12453 |
| Full Text |
|
| Is Part of |
FEBS Open Bio: 43rd FEBS congress biochemistry forever, Prague, Czech Republic, July 7‐12, 2018.. Hoboken, NJ : Wiley. 2018, vol. 8, suppl. 1, P.09-177-W, p. 264-265.. ISSN 2211-5463 |
| Keywords [eng] |
amorphous calcium phosphates ; tartaric or citric acid ; bone implants |
| Abstract [eng] |
Bone diseases significantly decrease the quality of human’s life, may be life-limiting and are recognized to be among the major health and social challenges of our time. The problems associated with application of auto- and allografts in current clinical practice stimulate the demand for improved new biomaterials for bone implants. The aim of our study was to evaluate the effect of newly synthesized amorphous calcium phosphates modifies with tartaric acid (ACP-TA) or (5% or 18%) citric acid (ACPCA-5, ACP-CA-18). Mouse (BALB/c 3T3 embryonic fibroblasts and cultures from bone marrow- BMC and bone explants- BEC) and human (Lep-3 and BJ embryonic fibroblasts, SAOS-2 osteosarcoma) cells were used as model systems in our investigations. Direct experiments (DE) with cells seeded on the surface of the materials were performed by MTT test and Scanning electron microscopy (SEM) after 3–6 days (MTT test) and 7–10 days (SEM). In indirect experiments (IDE) the cells were cultivated for 72 h in culture medium pre-incubated for 3, 6 and 21 days in the presence of the examined material and cell viability was estimated by MTT test. The results obtained demonstrate the ability of cells to adhere and proliferate on materials’ surface. Direct experiments with ACP-TA reveal cell viability ≥ 100% as compared to the control (BALB/c 3T3, BECs, Lep-3, SAOS-2). Longer pre-incubation period of culture medium with this material leads to increased cell viability (IDE). Viability of Lep-3 human fibroblasts estimated in both DE and IDE with ACP-CA-5 and ACP-CA-18 on 3rd day was ≥ 100% as compared to the control and then it starts to decrease but retains >70–80%. In contrast, the viability of human osteosarcoma SAOS-2 cells is around 50% in the same conditions. Better viability was observed with ACP-CA-5 than ACP-CA-18. In conclusion, among the materials investigated ACP-TA exhibit the most promising cytocompatibility proved in direct and indirect experiments with human and murine cells. |
| Published |
Hoboken, NJ : Wiley |
| Type |
Conference paper |
| Language |
English |
| Publication date |
2018 |
| CC license |
|
