| Abstract [eng] |
Currently, in the field of tissue engineering, much attention has been directed towards creation of polymeric scaffolds to aid tissue regeneration. The scaffolds have been constructed from both natural and synthetic polymers and immobilizing on them various compounds with antibacterial, antioxidant, and tissue regeneration-stimulating properties. In this work hyaluronic acid gel was prepared by cross-linking polymer using 1,4-butanediol diglycidyl ether. The gel blocks and granules were made and lyophilized in order to create porous structures. Active compounds possessing antibacterial, antiinflammatory, and tissue regeneration-stimulating properties were immobilized in the scaffolds. It was determined that when povidone iodine or chlorhexidine digluconate were immobilized in the block-shaped scaffolds, slow antibacterial substance release systems were created. The scaffolds with immobilized chlorhexidine digluconate exhibited antibacterial properties against Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, and Pseudomonas aeruginosa bacteria. Diameter of the bacterial growth inhibition area was reduced when low-molecular weight hyaluronic acid was added to the scaffolds. In vitro release kinetics of fibroblast growth factor from hyaluronic acid granules revealed a slow, sustained release, with a cumulative release of 32 % of the initial growth factor from the granules which included triblock copolymer. |
