| Abstract [eng] |
Sea buckthorn (Hippophae rhamnoides L.), widespread of the species genus, widely cultivated in Europe and Asia. Berry fruit of sea buckthorn has been utilized for juice and oil production including food additives to candies, jellies, jams, beverages and cosmetics and pharmaceuticals properties. Moreover, sea buckthorn leaves are used to produce leaf extracts, tea, tea powder or cosmetics. The aim of this study was to fractionate sea buckthorn pomace and leaves with the different polarity solvents, evaluate phytochemical composition, in vitro and ex vivo antioxidant activity of non – polar and polar constituents by using different assays. In the present study sea buckthorn pomace and leaves were extracted different extractions: Soxhlet extraction, solid-liquid extraction (SLE), pressurized liquid extraction (PLE) using nhexane, supercritical fluid extraction (SFE) using CO2. Sea buckthorn pomace and leaves solid residues after fluid extraction with CO2 has been applied for the solid-liquid (SLE) using four solvents of increasing polarity (acetone, ethanol, water, hydroethanolic 70/30 % mixture). Total phenolic content using Folin-Ciocalteu’s method, in vitro antioxidant activity was measured using ABTS•+, ORAC, HOSC, HORAC, also ex vivo cellular antioxidant (CAA) method. Solid residue antioxidant activity was measured with TPC and ABTS•+ scavenging capacity methods by approaching QUENCHER method. Non – polar sea buckthorn pomace and leaves SFE-CO2 extracts oxidative stability in rapeseed oil was measured with Oxipres method. Selected extracts cytotoxicity activity after 1 h incubation and cellular antioxidant activity were estimated using human colon adenocarcinoma model Caco-2 cell line. Phytochemical characterisation of chosen extracts was identified by UPLC-QTOF-MS, fatty acids composition was determined by GC-FID method. |
