| Abstract [eng] |
In this project it was determined that stator – rotor homogenizer may be used to disrupt Aphanizomenon flos – aquae biomass. Dissolved ammonium sulphate precipitate was filtered, at filtrate flux rate 999,1 l/ m2*h. 100ml protein solution was dialyzed with 10 l dialysis buffer to reduce solutions relative electrical conductivity from 134 to 1 mS/cm. diafiltration is used as a substitute to dialysis, it was measured that during the process average filtrate flux rate is 11,82 l/m2*h, diafiltration volume – 2,75. C – phycocyanin is stable in pH values from 3,5 to 7,5. It was determined that at static conditions C – phycocyanin binds to anion exchange resin at pH values that are higher than 5,2, for cation exchange resin objective protein binds at pH values that are lower than 5. At dynamic condition it was determined that optimal binding pH is 7, eluted C - phycocyanin purity (A620nm/A280nm) – 2,58. 43 ml 37,6 mg/ml protein concentration C – phycocyanin solution with (A620nm/A280nm) purity of 2,63 was produced from a larger scale anion exchange chromatography. Using capillary electrophoresis, it was determined that C – phycocyanin submit molecular weights are 16,6 and 21 kDa. Using size exclusion chromatographs, it was determined that C – phycocyanin submit molecular weights are 14 and 21 kDa. |
