| Abstract [eng] |
Diatom, Phaeodactylum tricornutum, was cultivated with various copper and iron concentrations. Copper and iron stress on Phaeodactylum tricornutum growth, changes in antioxidant activity and phenolic content were examined. Very high concentrations of copper and iron inhibited growth of Phaeodactylum tricornutum, lower but still relatively high concentrations of copper and iron negatively affected growth, but increased antioxidant activity and phenolic content of cultivated biomass. Diatoms Phaeodactylum tricornutum and Cylindrotheca closterium were cultivated with different glycerol concentrations. Their ability to grow under mixotrophic conditions were tested, changes in growth, antioxidant activity, phenolic content, fatty acid profile were examined. Phaeodactylum tricornutum and Cylindrotheca closterium were able to grow under mixotrophic conditions, glycerol addition promoted growth of both diatoms, with that antioxidant activity, phenolic content and fatty acid content were increase in both diatoms also. Phaeodactylum tricornutum pressurized liquid extraction (PLE) with three increasing polarity solvents (hexane, acetone, ethanol) at three temperatures (40oC, 80oC, 120oC) and three extraction times (15min, 39min, 60min) were performed. Total yield, EPA and DHA content in lipophilic fractions obtained by pressurized liquid extraction under various experimental conditions were examined. Total yield. total phenolic content (TPC) and in vitro antioxidant activity as measured by the ABTS+ assay in polar fractions obtained by pressurized liquid extraction under various experimental conditions were examined. Manufacturing process technology scheme for production of Phaeodactylum tricornutum extract using pressurized liquid extraction (PLE) was proposed. |
