| Abstract [eng] |
Xanthan has a wide application in industry. The aim is to optimize its synthesis while its demand is on the rise. This biopolimer is made during aerobic fermentation from Xanthomonas campestris bacteria. Xanthan yield depends on bacterial strain, carbon and nitrogen source, pH, temperature and chosen production process. For this assay xanthan production was optimized by changing nutritional elements of YM–T medium or adding additional nutrients. The biggest xanthan yield was noticed when fructose was the source of carbon, peptone from meat – the source of nitrogen, lactose with 3 g/l yeast extract – carbon and nitrogen combination, medium supplement – L-glutamic acid. Additionaly, xanthan was determined to have antioxidant properties by inhibiting DPPH radical. The antioxidant activity increased over time. Assays with linoleic acid for determining antioxidant activity showed, that with the increasing of biopolimer concentration, antioxidant properties also increased. pH and temperature also had influence on antioxidant properties. |
