| Title |
Coupled PCL hydrolysis and cellulose acetate deacetylation in cryo-coaxial core-shell fibres for wettability and TGF-β3 release control |
| Authors |
Bagdoniene, Lauryna ; Pupiute, Aiste ; Bagdonas, Edvardas ; Bernotiene, Eiva ; Ciuzas, Darius ; Gasiulyte, Eidvylė ; Baniukaitiene, Odeta ; Martuzevicius, Dainius ; Krugly, Edvinas |
| DOI |
10.1016/j.rechem.2026.103326 |
| Full Text |
|
| Is Part of |
Results in chemistry.. Amsterdam : Elsevier. 2026, Early access, p. 1-12.. ISSN 2211-7156 |
| Keywords [eng] |
Alkaline hydrolysis treatment ; Controlled TGF-β3 release ; Enhanced surface properties ; Fibrous scaffolds |
| Abstract [eng] |
Porous poly(ε-caprolactone)-hydroxyapatite/cellulose acetate (PCL-HAP/CA) core-shell fibrous scaffolds were fabricated by cryo-coaxial electrospinning and post treated by controlled alkaline hydrolysis to modify their surface chemistry and wettability. This treatment induced partial hydrolysis of the PCL ester bonds and deacetylation of CA, thereby increasing the number of hydroxyl and carboxyl groups on the scaffold surface. These changes were evaluated by Fourier transform infrared spectroscopy and Toluidine Blue O assay. The scaffold morphology and core-shell structure were analysed using scanning and transmission electron microscopy, whereas the thermal, structural, and mechanical changes were assessed using thermogravimetric analysis, differential scanning calorimetry, X-ray diffraction, and tensile testing. Alkaline hydrolysis markedly improved scaffold hydrophilicity, decreasing the water contact angle from 94.7 ± 2.8° to 16.5 ± 5.1° after 25 min of treatment, and increased phosphate buffered saline uptake. The fibrous structure was preserved after shorter treatment times, whereas prolonged treatment induced local fibre damage. After loading with transforming growth factor β3, the alkaline hydrolysis treated scaffolds showed a lower day-1 burst release than the untreated scaffolds (30.7% and 39.4%, respectively) and maintained gradual release over 7 days. These results show that alkaline hydrolysis can be used as a post treatment method to increase the surface functionality and hydrophilicity of cryo-coaxially electrospun PCL-HAP/CA core-shell scaffolds and to reduce the initial burst release of TGF-β3. |
| Published |
Amsterdam : Elsevier |
| Type |
Journal article |
| Language |
English |
| Publication date |
2026 |
| CC license |
|
