Title Screening of natural deep eutectic solvents for selective extraction of antioxidant rich fractions from Paeonia spp
Authors Abbas, S ; Dos Santos L., Cristina ; Venskutonis, P.R ; Ibáñez, E
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Is Part of 3rd COST CA22134 FoodWaStop meeting “Sustainable network for agrofood loss and waste prevention, management, quantification and valorisation”: 5-6 February 2025, Zadar, Croatia: abstract book.. Zadar : University of Zadar. 2025, P4.30., p. 75
Keywords [eng] Hansen solubility parameters ; Paeonia officinalis ; Paeonia anomala ; antioxidant activity ; NADES
Abstract [eng] This study applied the Hansen Solubility Parameter (HSP) model to screen and evaluate Natural Deep Eutectic Solvents (NADES) for the selective extraction of antioxidant-rich fractions from Paeonia officinalis and Paeonia anomala leaves and roots. Four NADES namely; N1 (Choline Chloride:Lactic Acid), N2 (Choline Chloride:Acetic Acid), N3 (Choline Chloride:Citric Acid), and N4 (Choline Chloride:Propylene Glycol) along with propylene glycol (PG) were selected based on predicted solubility affinity (Ra values) with methyl gallate. Ultrasound-assisted probe extraction (5 min, 35% amplitude) was used to obtain crude extracts, followed by solid-phase extraction (SPE) for NADES removal and purification. Extracts were analyzed for total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activity using ABTS and ORAC assays. Pressurized Liquid Extraction (PLE, 75% ethanol) served as a reference method. N4 exhibited the highest TPC for P. anomala leaves and roots of both species, while PLE yielded the highest TPC for P. officinalis leaves. TFC results indicated N2 as the most effective solvent. N4 showed superior ABTS radical scavenging activity across most samples, whereas ORAC trends varied by species. Overall, N4 and N2 provided the most selective antioxidant-rich extracts, supporting the predictive reliability of the HSP model. These findings demonstrate the potential of NADES as sustainable, selective alternatives to conventional solvents and advanced extraction techniques such as PLE.
Published Zadar : University of Zadar
Type Conference paper
Language English
Publication date 2025