| Title |
Microbial hydrogen production from depleted hydrocarbon reservoirs: evidence from field investigations and lab experiments |
| Authors |
Verma, Apoorv ; Pal, Mayur ; Vishwanath, R.S ; Kurkuri, Mahaveer ; Upendranath, K |
| DOI |
10.1016/j.egyr.2025.11.103 |
| Full Text |
|
| Is Part of |
Energy reports.. Amsterdam : Elsevier. 2025, vol. 14, p. 5616-5627.. ISSN 2352-4847 |
| Keywords [eng] |
Hydrogen production ; Metabarcoding ; Depleted hydrocarbon fields ; Anaerobic basal medium (ABM) ; Microbial processes |
| Abstract [eng] |
The study explores microbial Hydrogen (H₂) production in depleted hydrocarbon fields through field investigation and controlled experimental analyses (microbial process) using in-situ microbes, which thrive in thermophilic anerobic conditions of the hydrocarbon reservoirs. Gas and brine samples from various wells revealed H₂ presence in the range of 2–20 %, indicating microbial H₂ production potential in depleted hydrocarbon fields. Brine samples were characterized for their geochemical properties. Metabarcoding identified Clostridium as the most efficient H₂ producer, with other microbes including Hydrogenophaga, Desulfovibrio, Geobacter, and Thermomicrobium. In laboratory experiments, microbial cultures produced considerable hydrogen when grown in an anaerobic basal medium (ABM) enriched with 10 % v/v brine and 1 % v/v oil. The addition of oil significantly improved both the growth of the microbes and their hydrogen yield, providing valuable insights into the mechanisms of microbial hydrogen generation in hydrocarbon reservoirs. |
| Published |
Amsterdam : Elsevier |
| Type |
Journal article |
| Language |
English |
| Publication date |
2025 |
| CC license |
|
