Title New insights into secondary metabolite potential in hypericum perforatum, UPLC-ESI-MS/MS to novel phytochemical discovery with bioactivity assessment
Authors Roumaissa, Labza ; Cherif, Hamida Saida ; Derbak, Larbi ; Venskutonis, Petras Rimantas ; Demirtas, Ibrahim ; Mohammed M., Alzahrani ; Boufahja, Fehmi ; Bendif, Hamdi ; Garzoli, Stefania
DOI 10.1002/slct.202503590
Full Text Download
Is Part of ChemistrySelect.. Weinheim : Wiley-VCH. 2025, vol. 10, iss. 40, art. no. e03590, p. 1-17.. ISSN 2365-6549
Keywords [eng] Biological activities ; Hypericum perforatum ; In silico ADME ; LC-ESI-MS/MS ; Phenolic compounds
Abstract [eng] Different anatomical components of Hypericum perforatum (H. perforatum) have been utilized by humans for generations as a natural remedy with pharmacological attributes. This work aimed to investigate the secondary metabolite potential of H. perforatum from algeria using LC-ESI-MS/MS to determine its phytochemical profile. LC-ESI-MS/MS analysis revealed 22 components, with isoquercitrin being the most abundant bioactive compound at a concentration of 4162 µg/g. The total phenolic and flavonoid contents were also quantified, with TPC equal 171.54 ± 0.79 mg GAE/g and TFC equal 144.26 ± 14.3 mg QE/g. The antioxidant capacity of the extract was evaluated using in vitro assays, showing strong activity with an ABTS IC50 of 0.173 mg/mL. The antiproliferative potential of the methanolic extract was assessed by the MTT assay on CAPAN-1, DLD-1, and the healthy L929 cell line. The extract exhibited significant antiproliferative effects on CAPAN-1 and DLD-1 cells, with IC50 values of 0.807 ± 0.06 mg/mL and 0.953 ± 0.03 mg/mL, respectively, whereas no cytotoxicity was observed on L929 cells. Furthermore, SwissADME was used to evaluate the pharmacokinetics and drug-likeness of the main compounds. These findings enhance the understanding of H. perforatum and may support its prospective applications in pharmaceutical and cosmetic industries.
Published Weinheim : Wiley-VCH
Type Journal article
Language English
Publication date 2025
CC license CC license description