||Oilseed rape (Brassica napus L.) is one of the most important oil plants with a high content of oil. Rapeseed oil, which is free of anti-nutritional substances, is denoted by a higher dietary value than many other vegetable oils due to the low content of saturated fatty acids, high oleic acid content, favorable ratio of polyunsatured linoleic and linolenic acids, as well as the presence of sterols and fat-soluble vitamins. The productivity and growth of plants is affected by various abiotic factors, such as high or low temperatures, salinity, etc. Oilseed rape growth and yield quality could be enhanced by fertilizing, treating with growth regulators during growth. The number of pods per plant, the number of seeds per pod and the weight of seeds are important for the yield of oilseed rape. Their development is affected by nutrients as well as by hormones. In this thesis the effect of various concentrations of N-substituted β- and γ-amino acids with aromatic and azole moieties on the growth of oilseed rape (Brassica napus L.) in vitro was investigated for the first time; also, compounds having a positive effect on further field experiments have been determined. The field experiments were carried out with the most active compounds from the two different classes by growing spring oilseed rape and estimating the effect on the biometric parameters of spring oilseed rape, its yield as well as the seed and oil chemical composition.