| Abstract [eng] |
The topic of the Master's thesis is „Studies of collagen-based films with embedded bioactive substances". The aim of the project is to investigate the suitability of various compositions from collagen hydrolysis products, plasticizers and bioactive substances for obtaining films, and to determine their physical, mechanical and microbiological properties after their manufacture. Collagen and its derivatives are compatible with human skin, therefore they are often used in the medical industry. This work describes the main properties, extraction and application of collagen, gelatin and collagen hydrolysate. Films of various compositions with collagen hydrolysate, gelatin and different plasticizers (PEG 1500, PEG 20000, PEG 100000, glycerol) are produced, their organoleptic properties are evaluated and mechanical and physical tests are performed. It was determined that the most suitable composition for protein-based films is using collagen hydrolysate and gelatin, in a ratio of 1:1 and incorporating the plasticizer polyethyleneglycol (molecular weight 20000 g/mol). After determining the most suitable composition of the films – when the protein to plasticizer ratio is 2:3. Using this composition, synthetic and natural bioactive substances are incorporated: triazole 7-20, preservative Euxyl® 9010 PE, extract obtained from fireweed (lot. Chamaenerion angustifolium L.) and extract obtained from St. John's wort (lot. Hypericum perforatum L.). Mechanical, physical and microbiological tests are performed with these films, comparing how different bioactive substances change the properties of the film. The thermostability and chemical composition of all films are also evaluated and compared. Based on the conducted research and data presented in the literature, the most suitable and best-performing composition of the film with proteins, polyethyleneglycol and bioactive substance is determined, and a principal technological scheme was suggested. |
