| Abstract [eng] |
The biological activity of compounds shows their influence on biological, physiological and metabolic processes. Thus, biological activity is described as a property of a substance that depends on its structure, physical and chemical properties [1]. Hydrazides are a class of organic compounds that exhibit biological activity, e.g. antibacterial, antituberculosis, antifungal, anticancer, antiinflammatory, etc. Due to these properties, they are increasingly being studied for application in the pharmaceutical, cosmetic and agricultural industries, where effective and safe alternatives are sought for the production of various products. The main objective of this study was to determine and evaluate the antioxidant and antibacterial properties of hydrazide compounds, to select the compounds with the most intense, already listed properties, to supplement the nutrient medium for broccoli growth with them in vitro, and to determine the influence of the compounds on the antioxidant and antibacterial properties of broccoli. Based on the data, it was determined that the most intense antioxidant activity was observed in the compound 3,3'-((4-ethoxyphenyl)azanediyl)bis(N-(1,3-dioxoisoindolin-2-yl)propanamide), while the antibacterial activity was observed in 3,3’-((4-methoxyphenyl)azanediyl)bis(N’-(4-nitrobenzylidene)propane hydrazide)) and 3,3’-(phenylazanediyl)bis(N-(1,3-dioxoisoindolin-2-yl)propanamide). The results of the study showed that the compound 3,3’-((4-ethoxyphenyl)azanediyl)bis(N-(1,3-dioxoisoindolin-2-yl)propanamide) present in broccoli in vitro in the MS system stimulated the formation of secondary metabolites and had the highest antioxidant activity. It was found that the most intense antibacterial properties were found in broccoli grown in vitro in MS medium with 3,3’-(phenylazanediyl)bis(N-(1,3-dioxoisoindolin-2-yl)propanamide). The concentrations of chlorophyll a and b, carotenoids, L-proline and glucosinolates were evaluated during the study and it was found that the highest concentration of these compounds were induced by 3,3’-((4-ethoxyphenyl)azanediyl)bis(N-(1,3-dioxoisoindolin-2-yl)propanamide in MS medium. After analyzing the formation of phenolic compounds, (3,3’-(phenylazanediyl)bis(N-(1,3-dioxoisoindolin-2-yl)propanamide had the most significant effect on their formation. After evaluating the activity of superoxide dismutase, it was found that the biologically active compounds present in the MS broccoli nutrient in vitro medium did not have a significant effect on the antioxidant activity. Summarizing the obtained results, it can be concluded that broccoli grown in vitro on MS medium with the biologically active compound 3,3'-((4-ethoxyphenyl)azanediyl)bis(N-(1,3-dioxoisoindolin2-yl)propanamide) was the most suitable combination to stimulate the formation of secondary metabolites in broccoli. |
