| Abstract [eng] |
Fenugreek (Trigonella foenum-graecum L.) is a natural herbal plant which has several health benefits, antioxidant activity, anti-inflammatory, anti-diabetic, anti-carcinogenic properties and consumed all around the world since the ancient times. It has been used in folk medicine to lowering level of cholesterol in the bloodstream and reducing diabetes. Fenugreek seed is a great source of protein, dietary fibre, vitamin, mineral, edible oil, and bioactive components and mostly used for food consumption. However, to separate components from the fenugreek seed might be more beneficial to use it for various purposes such as food supplement, the source of natural medicine, media for the microorganism growth, and use in animal diet. Therefore, the aim of this work to obtain various fractions from the fenugreek seed to analyse their fractions and properties. Four major fractions were obtained respectively: fenugreek ethanol fraction (FEF), fenugreek oil fraction (FOF), fenugreek fermented fraction (FFF), and fenugreek powdered fraction (FPF). The main processes were applied in the following order in appropriate terms and conditions: extraction of fenugreek seed with ethanol, Supercritical Fluid Extraction-Carbon dioxide (SFE-CO2) extraction to obtain oil, fermentation application on modified fraction using with Lactobacillus casei microorganisms and lyophilization (freeze drying) process to obtain powdered fractions. Chemical compositions, total phenolic content, antioxidant activity, fatty acid composition, GC-MS analysis and microorganism determination were done for the fractions with the appropriate method used. Several compounds which possess antioxidant properties were identified in FEF. An increase in the number of microorganisms was observed in FFF. It was investigated characteristic of fenugreek oil composition. In general, all fractions evaluated and discussed at different points of their properties. |
