| Abstract [eng] |
Various types plant oily seeds accumulate bioactive compounds like vitamins, fatty acids, tocopherols and antioxidants. Many of these compounds are found in the oil, but also many of these bioactive compounds remain in seed cakes. Camelina (lot. Camelina sativa) is a plant, which ripen seeds containing up to 40 % of oil and is a natural source of Omega-3 and Omega-6 fatty acids. The aim of Master’s study is to isolate functional compounds from camelina seeds using non-conventional and conventional extraction methods. Chemical analysis of extracts and also evaluation of camelina solid residues and extracts antioxidant properties. Extracts from the camelina seeds were isolated using traditional and modern extraction techniques such as Soxhlet extraction, supercritical CO2 extraction, accelerated solvent extraction. Response Surface Methodology was used for the optimization of accelerated hexane extraction to set variable parameters (time, temperature, solvent volume) giving the maximum yields of lipophilic extracts. After supercritical CO2 extraction obtained residue were extracted by accelerated solvent extraction with different polarity solvents. And also enzymatic hydrolysis were done. Antioxidant activity of extracts were measured using DPPH• radical and ABTS•+ radical fading methods. Also oxygen radical absorbance capacity (ORAC) were evaluated using ORAC method. Total phenolic compounds amounts were determined by Folin-Ciocalteu‘s method. Solid residues antioxidant activity were estimated by previously reported analysis methods using QUENCHER method. Fatty acids composition of lipophilic extracts were analyzed by gas chromatography, tocopherol content identified by high performance liquid chromatography and preliminary bioactive compounds evaluation was performed by ultra high performance liquid chromatography. Oxidative stability of lipophilic extracts was measured using Oxipres method. |
