Title Modeliavimo aplinkos, biotechnologinių procesų analizei ir tyrimams, sukūrimas /
Translation of Title Development of modeling environment for bioprecesses analysis and investigation.
Authors Baranskas, Deividas
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Pages 77
Keywords [eng] modeling of growing yeast ; modeling of growing E.coli bacteria ; modeling of growing mammalian cells culture ; graphical modeling environment
Abstract [eng] In this work there is conducted analysis of the literature about technological peculiarities of important biotechnological processes (yeast growing, E.coli bacteria and mammalian cells culture) and mathematical modelling principles of these processes. It is presented yeast classification, phases of their growth and the mathematical model. It is analysed aspects of E.coli bacteria culture, biology of bacteria and mathematical model of growth. It is described biotechnological processes of mammalian cells growing and mathematical modelling of these processes. It is presented classification of the bioreactors. Mathematical models of culturing all three cultures are implemented in the Matlab/Simulink environment. For the realization of each above-mentioned model it is created a user-friendly graphical interface. It is made schemes for the regulation of important culture process variables, which allows modelling various culture regimes for the user oneself. Model of yeast growing process is used for regulating dissolved oxygen concentration in the medium, changing the coefficient of the mass transfer from gaseous to the liquid (by manipulating the air flow and the stirrer speed). E.coli growing process model is used to illustrate the control principle of glucose concentration in the reactor while changing glucose feeding stream. Process model of growing mammalian cells is used for regulation of glutamine concentration in the medium while changing glutamine feeding stream. Results of modelling are presented under various culture conditions which can be chosen by the user. At the end of work there are presented results of the work and conclusions.
Dissertation Institution Kauno technologijos universitetas.
Type Master thesis
Language Lithuanian
Publication date 2017