| Abstract [eng] |
This annotation outlines the application of the Nano/micro composite fibrous filter for sampling aerosol particles in aerosol particle cytotoxicity studies. The research involves the production and use of a specialized sampling filter. This filter is characterized by a layered composite arrangement of nano- to sub-micro-meter-sized polymeric fibers. It enhances particle retention efficiency and mechanical stability while allowing for a range of subsequent physico-chemical or toxicological analyses. The filter features a composite matrix with a nanofibrous top layer designed to capture aerosol nanoparticles effectively. This top layer provides a high surface area for particle deposition and ensures that particles are retained securely. The underlying matrix supports mechanical stability and facilitates cell cultivation. This dual functionality of particle collection and cell scaffolding is a notable advancement over traditional particle sampling methods. In the described method, aerosol particles are collected directly onto the filter, preserving their original form and characteristics. After collection, the filter is immersed in culture media, and cells are seeded directly onto the particles. This setup allows for direct interaction between the cells and the particles, providing a more accurate assessment of cytotoxicity compared to indirect methods where particles are dissolved in culture media. The cytotoxicity of aerosol particles is evaluated based on monitoring cellular health indicators, including cell viability, stress responses, and other biomarkers. This direct exposure of cells to the particles on the filter surface ensures a more realistic representation of particle toxicity. The method offers several advantages. It improves accuracy by providing a direct interaction between cells and particles, better reflecting the particles’ toxic potential. The approach enhances efficiency by eliminating several procedural steps, such as particle extraction and dissolution, which are common in traditional methods. This simplification reduces both the time and resources required for testing. Additionally, the method is cost-effective due to lower material use and reduced reliance on solvents, contributing to a decreased environmental impact. The applicability of this method extends across various environments, including indoor occupational settings, residential areas, hospitality venues, and outdoor air. It provides a versatile tool for assessing the cytotoxicity of aerosol particles in different contexts, offering valuable data for research and public health monitoring. In summary, the “nano/micro composite fibrous filter” and its application in cytotoxicity assessment represent a significant advancement in aerosol particle analysis. The integration of particle collection and cell cultivation on a single platform enhances both the accuracy and efficiency of toxicity testing. This approach offers a more reliable and streamlined process for understanding the health impacts of airborne particulate matter, contributing to improved public health outcomes and more informed regulatory measures. |
