| Title |
Transcription factor-based biosensors for detection of naturally occurring phenolic acids / |
| Authors |
Augustiniene, Ernesta ; Kutraite, Ingrida ; Valanciene, Egle ; Matulis, Paulius ; Jonuskiene, Ilona ; Malys, Naglis |
| DOI |
10.1016/j.nbt.2023.09.004 |
| Full Text |
|
| Is Part of |
New biotechnology.. Amsterdam : Elsevier. 2023, vol. 78, p. 1-12.. ISSN 1871-6784. eISSN 1876-4347 |
| Keywords [eng] |
whole-cell biosensor ; transcription factor ; inducible gene expression system ; phenolic acid |
| Abstract [eng] |
Phenolic acids including hydroxybenzoic and hydroxycinnamic acids are secondary plant and fungal metabolites involved in many physiological processes offering health and dietary benefits. They are often utilised as precursors for production of value-added compounds. The limited availability of synthetic biology tools, such as whole-cell biosensors suitable for monitoring the dynamics of phenolic acids intracellularly and extracellularly, hinders the capabilities to develop high-throughput screens to study their metabolism and forward engineering. Here, by applying a multi-genome approach, we have identified phenolic acid-inducible gene expression systems composed of transcription factor-inducible promoter pairs responding to eleven different phenolic acids. Subsequently, they were used for the development of whole-cell biosensors based on model bacterial hosts, such as Escherichia coli, Cupriavidus necator and Pseudomonas putida. The dynamics and range of the biosensors were evaluated by establishing their response and sensitivity landscapes. The specificity and previously uncharacterised interactions between transcription factor and its effector(s) were identified by a screen of twenty major phenolic acids. To exemplify applicability, we utilise a protocatechuic acid-biosensor to identify enzymes with enhanced activity for conversion of p-hydroxybenzoate to protocatechuate. Transcription factor-based biosensors developed in this study will advance the analytics of phenolic acids and expedite research into their metabolism. |
| Published |
Amsterdam : Elsevier |
| Type |
Journal article |
| Language |
English |
| Publication date |
2023 |
| CC license |
|
