| Abstract [eng] |
The importance of controlling the activity of many enzymes has long been proven in medical science. Inhibition of certain hydrolase enzymes is recognized to be important in modulating physiological functions in the human body in cases of carbohydrate metabolism disorder, dementia, high blood pressure and some other health problems. It is also acknowledged that the effective use of phytochemicals in medicinal and other plants with inhibitory effects can significantly contribute to the prevention, risk reduction, and complex therapy of these negative physiological functions. Studies conducted on the phytochemical composition and biological effects of plant raw materials can be used to enrich the assortment of medicinal plant raw materials. In this doctoral thesis, investigates phytochemical composition of D. caucasica, D. nipponica and A. glycyphyllos extracts and their ability to inhibit of physiologically important enzymes belonging to family of hydrolases. By applying biological methods, evaluated the effect of D. caucasica, D. nipponica and A. glycyphyllos extracts on hydrolase enzymes activities and theoretically formulated assumption that extracts contain natural compounds capable of binding with enzymes exhibiting hydrolase activity and thus reduce their activity. This thesis provides insights, that the different anatomical parts of the plants D. caucasica, D. nipponica and A. glycyphyllos selected for the research may contain valuable phytochemical compounds that are little researched, which may be characterised by diverse bioactivity, including targeted effects on the activity of physiologically important enzymes – α-amylase, α glucosidase, angiotensin-converting enzyme and acetylcholinesterase. |
