| Abstract [eng] |
St. John's wort (Hypericum perforatum L.) accumulates numerous secondary metabolites that provide beneficial properties such as antidepressant, antioxidant, antibacterial, and others. In modern society, there is a search for means to replace synthetic compounds with naturally derived ones that possess similar beneficial qualities, and St. John's wort is an excellent source of various useful compounds. However, cultivating the plant in vivo and extracting targeted compounds is a long process that depends on various factors. One significant advantage of in vitro cultivation is the ability to standardize environmental factors, optimize conditions and plant growth hormones to obtain high yields of beneficial substances, significantly shortening the process from sowing to product extraction. This work investigates the influence of plant growth hormones on the in vitro cultivation of St. John's wort, evaluating the antioxidant activity, concentrations of phenolic compounds, phenolic acids, flavonoids, anthocyanins, chlorophylls, as well as carotenoids, proteins, and some antioxidative enzymes in cultures of St. John's wort induced by plant growth hormones. Three extracts obtained from St. John's wort cultures grown in MS medium with plant growth hormones were used for the research: 0.5 mg/l TDZ and 0.1 mg/l IAA; 0.11 μM kinetin and 0.9 μM 2,4-D; 0.1 mg/l NAA, 0.2 mg/l BAP, and 0.5 mg/l 2,4-D. The recommendation section provides a scheme for obtaining hypericin which could be applied and optimized in the industry by manipulating the combination of plant growth hormones. |
