Title Iš dirvožemio išskirtų Bacillus spp. bakterijų tyrimai ir jų antimikrobinio poveikio prieš augalų patogeninius grybus įvertinimas /
Translation of Title Studies of Bacillus spp. bacteria isolated from soil and evaluation of their antimicrobial activity against plant pathogenic fungi.
Authors Pečiulis, Kristijonas
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Pages 62
Keywords [eng] Bacillus spp. ; bacteria ; plants ; inhibition ; pathogens
Abstract [eng] As the human population grows, the demand for food increases. In order to meet the growing demand for food, agricultural capacity needs to be enhanced.The pursuit of higher crop yields requires greater use of pesticides, which negatively impacts both the environment and human health. An alternative solution is biopesticides, which are safe for the environment, human and animal health. In our research, it was investigated whether bacteria isolated from the soil can become an alternative to synthetic pesticides and inhibit plant phytopathogens while promoting plant growth as well. Six reference Bacillus spp. bacterial cultures (B. cereus (B1), B. circulans (B2), B. coagulans (B3), B. megaterium (B4), B. subtilis (B5), B. sphaericus (B6), together with six from soil isolated bacteria (022-3 (B7), 022-4 (B8), 2-1 (B9), 4-2 (B10), 8-1 (B11), 8-2 (B12)) were tested as inhibitors to fitopathogens. Bacteria isolated from the soil were tested on their inhibition intensity by incubating at 25 ⁰C and 37 ⁰C. Bacteria isolated from soil were used in plant growth promotion studies. In our study, bacterial cultures inhibited the phytopathogenic fungi Fusarium avenaceum, Fusarium graminearum 4 and Fusarium graminearum 13121 most effectively. When incubating the samples at temperatures of 25 ⁰C and 37 ⁰C, it was found that the bacterial culture isolated from soil 8-2 used in sample B12 had the greatest influence on phytopathogenic fungi at lower temperatures, in another study, the temperature of 37 ⁰C had the greatest influence on phytopathogenic fungi. Applying the suspensions of bacteria isolated from the soil to plant seeds, the number of sprouts and leaves of the plant was increased, and its total weight was higher, but the stem and root lengths were smaller, and the number of dried leaves was increased.
Dissertation Institution Kauno technologijos universitetas.
Type Master thesis
Language Lithuanian
Publication date 2023