| Abstract [eng] |
Because of the large agricultural harvest losses and the adverse effects of chemical pesticides, alternative and effective methods of biocontrol of pathogens are being sought. One alternative option is using biological plant protection products such as essential oils or extracts. These products contain biologically active substances that can control horticultural crop pathogens. This work aims to assess carrot pathogens population diversity and species composition and evaluate the effect of biologically active substances on the Alternaria spp. The diversity of microorganisms in the carrot crop was assessed by the agarized media method on different plant hosts: Galinsoga parviflora, Equisetum arvense, Tripleurospermum perforatum, and carrot roots. Bacteria, yeasts and fungi of different genera were found on the investigated plant hosts: Alternaria, Penicillium, Mucor, Botrytis, Fusarium, etc. After assessing the infection, selected monocultures of microscopic fungi isolated and identified by molecular methods. The following species of microscopic fungi from different plant-hosts evaluated in efficacy and reinoculation experiments: Alternaria alternata (ALT1 and ALT2), A. botrytis (ALT3), A. consortialis (ALT4). Thyme (Thymus vulgaris L.) essential oil at a concentration of 100 μl/l and clove (Syzygium aromaticum L.) extract at different concentrations were used in the experiments. It was found that thyme essential oil at a concentration of 100 μl/l had an inhibitory effect against Alternaria spp. ALT1, ALT2, ALT4 isolates from different plant hosts. Clove extract inhibited only ALT1 and ALT4 isolates. Our research showed that biologically active substances can inhibit the development of microscopic fungi. |
