| Title |
Biorefining of three Vincetoxicum species into valuable components by different extraction methods / |
| Authors |
Jovaišaitė, Jovita ; Jūrienė, Laura ; Pukalskas, Audrius ; Venskutonis, Petras Rimantas |
| DOI |
10.15388/IOR2023 |
| ISBN |
9786090708835 |
| Full Text |
|
| Is Part of |
Open readings 2023: 66th international conference for students of physics and natural sciences, April 18-21, 2023, Vilnius, Lithuania: annual abstract book / editors: M. Keršys, Š. Mickus.. Vilnius : Vilnius University press, 2023. p. 136.. ISBN 9786090708835 |
| Abstract [eng] |
Vincetoxicum belongs to the family Apocynaceae, the subfamily Asclepiadoideae [1]. It is an herbaceous, perennial, climbing plant native to Europe, North America and Asia. In Chinese medicine, the plant has often been used to treat scabies. Additionally, swallow-wort have diuretic and laxative properties and a powder made from the roots is said to accelerate wound healing. It must be stressed that swallow-wort is a poisonous plant, which in large doses can affects the nervous, vascular and cardiac systems [2]. However, there is very little detailed scientific information on the phytochemical composition and properties of these plants. The aim of this work was to develop technology for producing various fractions rich in bioactives from three Vincetoxicum species using biorefining concept and characterize the extracts. V. hirundinaria, V. nigrum and V. luteum were defatted by supercritical fluid extraction with carbon dioxide (SFE-CO2). The lipophilic extracts were analyzed by different methods. Triacylglycerols (TAGs) were analyzed by ultra-performance liquid chromatography (UPLC), while the total chlorophyll and carotenoid were measured by spectrophotometric method. Defatted leaves were further extracted by the mixture of three different polarity solvents (acetone-methanol-water). Antioxidant activity of non-polar and polar extracts was evaluated by using different in vitro antioxidant capacity assays: the DDPH assay and the Oxygen Radical Absorbance Capacity (ORAC) assay. Total phenol content was measured by Folin-Ciocalteu method. The phytochemical composition of V. luteum extract was analysed by ultra-performance liquid chromatography (UPLC). The yields of lipophilic fractions recovered by SFE-CO2 were 1.2-1.5%. Linoleic linolenic, oleic and palmitic acids were major in the extracted triacylglycerols. The concentration of carotenoids and chlorophylls varied between different species. The extracts obtained by different extraction methods were characterized by their antioxidant potential. Thus, all measured values for lipophilic fractions were rather low, while the mixture of different polarity solvents recovered the majority of polar polyphenolic antioxidants. UPLC-MS/MS results indicated that in V. luteum extract was identified several phenolic compounds: chlorogenic acid, caffeic acid, isoquercitrin, kaempferol and various flavonoid glycosides. The obtained extracts contain different classes of bioactive compounds. It can be concluded that extraction by supercritical fluid extraction with carbon dioxide and the mixture of different polarity solvents can extract valuable bioactive compounds from leaves of different Vincetoxicum species. |
| Published |
Vilnius : Vilnius University press, 2023 |
| Type |
Conference paper |
| Language |
English |
| Publication date |
2023 |
| CC license |
|
