| Abstract [eng] |
INTRODUCTION. Cancer is one of the major health challenges of our time, affecting millions of people around the world. It is a complex and multi-faceted disease that can affect people of all ages, genders, and ethnicities. Currently, cancer is treated with surgical resection, radiation therapy, and chemotherapy. Unfortunately, these treatments are often not successful and can cause serious side effects. In vitro scaffold adaptation is a new approach to treating cancer that could be more effective in treating the disease. This approach uses a scaffold made of inert materials such as polymers, hydrogels, etc., to provide a 3D structure that can be used to study the behavior of cancer cells. The scaffold provides a supportive environment for cancer cells, allowing them to grow and spread in a controlled manner. In addition, the scaffold can be adapted to provide a more targeted form of therapy, such as targeted drug delivery [1, 2]. METHODS. 3D fibrous polymer-based scaffolds were produced by 3D fibre printer (3Df-01C, Bious Labs, Lithuania, https://life.biouslabs.com/), which is a combination of melt electrospinning and fused deposition modelling [3]. To prevent cell migration away from the scaffold, a nanofibrous layer was added to the bottom of the scaffold via solution electrospinning. The hydrophilicity of the scaffolds was improved through lowtemperature plasma treatment. The cytotoxicity of the 3D fibrous scaffolds was evaluated according to ISO 10993-5:2009 using mouse fibroblast cells L929. Additionally, the scaffolds were cultivated with human breast cell line MDA-MB-231 and glioblastoma cell line U87-MG. RESULTS. Three different PCL scaffolds were produced with varying fiber and pore sizes, fiber width ranging between 8 and 25 μm, and pores between 35 and 155 μm (see Fig.1). After lowtemperature plasma treatment, the scaffolds were more hydrophilic, with the WCA 60 ± 5°. Results of the MTT test demonstrated that the viability of the human breast cell lines MDA-MB-231 and U87-MG was equal to or higher than that of the 2D cultures during the seven-day period. Moreover, the scaffold supported the cellular growth and viability effectively. Additionally, the MDA-MB-231 cells had a more elongated shape, which was closer to their natural form. [...]. |
