| Title |
ZnO tetrapods for label-free optical biosensing: physicochemical characterization and functionalization strategies |
| Authors |
Terracciano, Monica ; Račkauskas, Simas ; Falanga, Andrea Patrizia ; Martino, Sara ; Chianese, Giovanna ; Greco, Francesca ; Piccialli, Gennaro ; Viscardi, Guido ; De Stefano, Luca ; Oliviero, Giorgia ; Borbone, Nicola ; Rea, Ilaria |
| DOI |
10.3390/ijms24054449 |
| Full Text |
|
| Is Part of |
International journal of molecular sciences.. Basel : MDPI. 2023, vol. 24, iss. 5, art. no. 4449, p. 1-15.. ISSN 1422-0067 |
| Keywords [eng] |
label-free detection ; quantum yield ; surface characterization ; surface functionalization ; zinc oxide nanostructure |
| Abstract [eng] |
In this study, we fabricated three different ZnO tetrapodal nanostructures (ZnO-Ts) by a combustion process and studied their physicochemical properties by different techniques to evaluate their potentiality for label-free biosensing purposes. Then, we explored the chemical reactivity of ZnO-Ts by quantifying the available functional hydroxyl groups (-OH) on the transducer surface necessary for biosensor development. The best ZnO-T sample was chemically modified and bioconjugated with biotin as a model bioprobe by a multi-step procedure based on silanization and carbodiimide chemistry. The results demonstrated that the ZnO-Ts could be easily and efficiently biomodified, and sensing experiments based on the streptavidin target detection confirmed these structures' suitability for biosensing applications. |
| Published |
Basel : MDPI |
| Type |
Journal article |
| Language |
English |
| Publication date |
2023 |
| CC license |
|
