| Abstract [eng] |
Phytochemicals not only protect plants from biotic or abiotic stress, but are also used. in the pharmaceutical, food and agricultural industries. Most of .the medicines on the market are synthetic modifications of natural substances. At present, the growing interest of consumers in natural products is aimed at improving the production of bioactive compounds in plants, so various strategies are being researched more widely, and more efficient way of using these substances is being sought. Medicinal sage (Salvia officinalis L.) and peppermint. (Mentha piperita L.) synthesize a wide range of phytochemical substances that determine their bioactivity. In this work, the secondary metabolites of peppermint (Mentha piperita L.) and sage (Salvia officinalis L.) and the bioactivity of medicinal plant extracts were quantified. The total concentrations of phenolic compounds, flavonoids, phenolic acids, proteins, amino acids, carotenoids, ascorbic acid, anthocyanins and antibacterial activity against Xanthomonas campestris and Rhizobium radiobacter were tested in vitro and in vivo extracts. Antioxidant activity according to ABTS, DPPH, FRAP and reduction method as well as activity of antioxidant enzymes were also determined. The highest antioxidant potential of S. officinalis samples was in sage leaves in vitro cultures grown on MS medium with NAA (0.1 mg/l), BAP (0.2 mg/l) and 2,4-D. (0.5 mg/l). and the best extract of M. piperita was peppermint leaves and stems in vitro grown on MS medium. with BAP (1 mg /l). The highest concentrations of chlorophyll a and b, carotenoids, ascorbic acid, amino acids, antioxidant enzymes, phenolic compounds, flavonoids were found in the sage leaves callus culture extracts grown on MS media supplemented with NAA (0.1 mg/l), BAP (0.2 mg/l) and 2,4-D (0.5 mg/l), in addition, the results correlate with the investigated antioxidant capacity. The highest concentrations of phenolic acids and proteins were found in peppermint stem callus cultures grown on MS medium with BAP (1 mg/l), 2,4-D (0.2 mg/l) extract, and the highest concentration of anthocyanin was found in M. piperita leaf in vivo extract. Of the tested plants, the most effective antibacterial activity against Xanthomonas campestris and Rhizobium radiobacter was the sage leaf extract with a zone of inhibition –7.67 mm. The results obtained suggest that plants of Salvia officinalis L. and Mentha piperita L. may be used for the production of large-scale medicinal metabolites and may be antimicrobial or antioxidant agents, but this requires further investigation. |
