| Abstract [eng] |
The encapsulation of bioactive compounds in various polymeric matrices can help to overcome the disadvantages inherent in these materials. Often these compounds have inappropriate properties: uncontrolled release in the body, low water solubility or even toxicity. Electrospinning technology is used to solve these problems. In the electrospinning method, the functional compound is encapsulated in a polymeric matrix, which can improve its properties and help control release. There are no data in the literature on the encapsulation of the antiepileptic drug carbamazepine in the polylactic acid matrix, the chemical and mechanical properties of the fibers and the release kinetics, therefore the final master's project is dedicated to this study. During the research of the master's project the structure and properties of the formed fibers by electrospinning were analyzed. Scanning electron microscopy revealed the formation of an uneven surface and porous micro scaffolds that were randomly oriented. The maximum fiber diameter was measured on samples produced at 30 % relative humidity and the smallest at 60 % relative humidity. The densest pores are located on the scaffolds, which are produced at the highest relative humidity – 60 %, but the diameter of the pores decreased with increasing relative humidity. Fourier transform infrared spectroscopy revealed that the fibers had functional groups characteristic of polylactic acid and carbamazepine. Analysis of the water contact angle showed that the water contact angle depends on the amount of carbamazepine in the fiber. The hydrophobicity of the fiber increased with increasing carbamazepine amount. The tensile tests performed on the fibers showed that the fibers produced at 30 % relative humidity had the best mechanical tensile properties. It was observed that the tensile strength of the fibers decreased with increasing relative humidity. A study of the release kinetics of carbamazepine showed that a higher percentage of carbamazepine release could be achieved by increasing the relative humidity and the amount of carbamazepine in the sample. The drug was released most intensely up to 10 hours, and release slowed from 10 to 24 hours. Based on the obtained results, recommendations for the industrial production of fibers were provided. |
