Title Synthesis, biological activity, and molecular modelling studies of naphthoquinone derivatives as promising anticancer candidates targeting COX-2 /
Authors Kavaliauskas, Povilas ; Opazo, Felipe Stambuk ; Acevedo, Waldo ; Petraitiene, Ruta ; Grybaitė, Birutė ; Anusevičius, Kazimieras ; Mickevičius, Vytautas ; Belyakov, Sergey ; Petraitis, Vidmantas
DOI 10.3390/ph15050541
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Is Part of Pharmaceuticals.. Basel : MDPI. 2022, vol. 15, iss. 5, art. no. 541, p. 1-26.. ISSN 1424-8247
Keywords [eng] non-small cell lung cancer ; naphthoquinone ; ROS ; mitochondrial damage ; anticancer
Abstract [eng] Non-small cell lung cancer (NSCLC) remains a leading cause of cancer-associated mortalities worldwide. Therefore, it is crucial to develop a novel therapeutic option targeting localized and metastatic NSCLC. In this paper, we describe the synthesis and biological activity characterization of naphthoquinone derivatives bearing selective anticancer activity to NSCLC via a COX-2 mediated pathway. The biological evaluation of compounds 9–16 showed promising structure-dependent anticancer activity on A549 cells in 2D and 3D models. Compounds were able to significantly (p < 0.05) reduce the A549 viability after 24 h of treatment in comparison to treated control. Compounds 9 and 16 bearing phenylamino and 4-hydroxyphenylamino substituents demonstrated the most promising anticancer activity and were able to induce mitochondrial damage and ROS formation. Furthermore, most promising compounds showed significantly lower cytotoxicity to non-cancerous Vero cells. The in silico ADMET properties revealed promising drug-like properties of compounds 9 and 16. Both compounds demonstrated favorable predicted GI absorption values, while only 16 was predicted to be permeable through the blood–brain barrier. Molecular modeling studies identified that compound 16 is able to interact with COX-2 in arachidonic acid site. Further studies are needed to better understand the safety and in vivo efficacy of compounds 9 and 16.
Published Basel : MDPI
Type Journal article
Language English
Publication date 2022
CC license CC license description