| Title |
Kinetic and stoichiometric modeling-based analysis of do-cosahexaenoic acid (DHA) production potential by C.cohnii from glycerol, glucose and ethanol / |
| Authors |
Berzins, Kristaps ; Muiznieks, Reinis ; Baumanis, Matiss R ; Strazdina, Inese ; Shvirksts, Karlis ; Prikule, Santa ; Galvanauskas, Vytautas ; Pleissner, Daniel ; Pentjuss, Agris ; Grube, Mara ; Kalnenieks, Uldis ; Stalidzans, Egils |
| DOI |
10.3390/md20020115 |
| Full Text |
|
| Is Part of |
Marine drugs.. Basel : MDPI. 2022, vol. 20, iss. 2, art. no. 115, p. 1-18.. ISSN 1660-3397 |
| Keywords [eng] |
crypthecodinium cohnii ; docosahexaenoic acid ; Krebs cycle ; central metabolism ; kinetic model ; constraint-based model |
| Abstract [eng] |
Docosahexaenoic acid (DHA) is one of the most important long-chain polyunsaturated fatty acids (LC-PUFAs), having numerous health benefits. Crypthecodinium cohnii, a marine heterotrophic dinoflagellate, has been successfully used for industrial production of DHA because it can accu-mulate DHA at high concentrations within the cells. Glycerol is an interesting renewable substrate for DHA production since it is a by-product of biodiesel production and other industries, and globally is generated in large quantities. The DHA production potential from glycerol, ethanol and glucose have been compared by combining fermentation experiments with pathway-scale kinetic modeling and constraint-based stoichiometric modeling of C.cohnii metabolism. Glycerol has the slowest biomass growth rate among tested substrates. That is partially compensated by the highest PUFAs fraction where DHA is dominating. Mathematical modeling reveals that glycerol has the best experimentally observed carbon transformation rate into biomass, reaching the closest values to the theoretical upper limit. In addition to our observations, the published experimental evidence also indicates that crude glycerol is readily consumed by C.cohnii making glycerol an attractive substrate for DHA production. |
| Published |
Basel : MDPI |
| Type |
Journal article |
| Language |
English |
| Publication date |
2022 |
| CC license |
|
